rabbit polyclonal anti p62 (Santa Cruz Biotechnology)
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Structured Review
Santa Cruz Biotechnology
rabbit polyclonal anti p62
Rabbit Polyclonal Anti P62, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+usf+1/pmc06138730-153-16-28?v=Santa+Cruz+Biotechnology
Average 94 stars, based on 136 article reviews
Rabbit Polyclonal Anti P62, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+usf+1/pmc06138730-153-16-28?v=Santa+Cruz+Biotechnology
Average 94 stars, based on 136 article reviews
rabbit polyclonal anti p62 - by Bioz Stars,
2026-07
94/100 stars
Images
1) Product Images from "Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins"
Article Title: Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins
Journal: Nature Communications
doi: 10.1038/s41467-018-06275-1
Figure Legend Snippet: Fn14-positive vesicles accumulate in a TWEAK-dependent manner upon Atg8s inactivation by dominant-negative ATG4B. a – f WT or ATG4B DN stable expression HeLa cells were assayed as follows: a treated with TWK for indicated times, immunostained for Fn14 and TRAF2, and analyzed by confocal microscopy (scale bar, 20 µm); b treated with TWK and subjected to membrane floatation assay as detailed under Methods; c transfected with luciferase expression constructs as detailed under Methods, treated with TWK as indicated, and further subjected to luciferase assay as detailed to assess NF-kB activity (* P < 0.05, n = 3 biological repeats; comparisons by T test; mean ± s.e.m.). The vertical line represents control in the absence of TWK; d subjected to Proteinase protection assay (upper panel) as detailed under Methods and schematically depicted (lower panel). e , f transfected with si NT or siRNA against p62 (si p62 ) and treated with TWK, extracted proteins were immunoblotted ( e ) or cells were immunostained for Fn14 and analyzed by confocal microscopy (scale bar, 20 µm) ( f )
Techniques Used: Dominant Negative Mutation, Expressing, Confocal Microscopy, Membrane, Transfection, Luciferase, Construct, Activity Assay, Control
Figure Legend Snippet: Autophagy and MVBs both contribute to Fn14 trafficking. a – d WT or ATG4B DN stable expression HeLa cells were treated as follows and analyzed by confocal microscopy (scale bar, 20 µm): a treated with wortmannin as indicated and immunostained for Fn14 and p62; b transfected with si NT or siRNA against TRAF2 (si TRAF2 ), treated with TWK as indicated, and immunostained for Fn14. Arrows and arrowheads point at Fn14 at vesicles and plasma membrane, respectively; c , d transfected with si NT or siRNA against BECN1 (si BECN1 ) ( c ) or TSG101 (si TSG101 ) ( d ), treated with TWK, and immunostained for Fn14 and EEA1
Techniques Used: Expressing, Confocal Microscopy, Transfection, Clinical Proteomics, Membrane
Figure Legend Snippet: Regulation of Fn14 by the autophagic machinery. GABARAP-mediated autophagy is responsible for the main pool of the cellular Fn14 and in its absence the receptor accumulates in ERGIC. GATE-16-mediated autophagy and the canonical MVB pathway are responsible in parallel for degradation of Fn14 in endocytic compartments. In absence of GATE-16, Fn14-positive endosomes accumulate at the vicinity of open phagophores. Recruitment of Fn14 to either GABARAP- or GATE-16-dependent autophagosomes is mediated by the cargo receptor p62. Recruitment of internalized Fn14 through the GATE-16-dependent pathway requires TRAF2. The mechanism responsible for recruitment Fn14 to GABARAP-dependent autophagy is still unknown
Techniques Used:
